Induction of Nucleic Acid Damage in Viral Genomes Using Riboflavin in Combination with UV Light

نویسندگان

  • A Elikaei Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran
  • F Yari Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
  • H Latifi Laser and Plasma Research Institute, Shahid Beheshti University, Tehran, Iran
  • H Mirshafiee Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran
  • H Nikbakht Laser and Plasma Research Institute, Shahid Beheshti University, Tehran, Iran
  • SM Hosseini Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran
  • Z Sharifi Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
چکیده مقاله:

Background and Aims: Despite the screening of blood donors, blood transfusion represents an ideal port of entry for blood-borne infection. Blood-borne pathogen transmission has been a concern since the earliest days of transfusion. The blood product of platelet (PLT) concentrates is still faced with the risk of bacterial and viral contaminations. Pathogen inactivation technologies offer a proactive approach and the potential to further improve blood safety. Here we study the pathogen inactivating capacity of riboflavin with UV light treatment in platelet concentrates contaminated with enveloped and non-enveloped viruses. Materials and Methods: The inactivation effects of riboflavin in combination with UV light was examined on Herpes simplex virus (HSV), Vesicular stomatitis virus (VSV) and Polio virus classified as enveloped and non-enveloped DNA and RNA viruses, respectively. After spiking viruses in PLT concentrate, treatment was undertaken with riboflavin (50 µM) and exposed to different doses of UV light. Residual viral infectivity was titrated using 50%Tissue Culture Infective Dose (TCID50). Results: Combination of Riboflavin and UV light treatment reduced the titer of HSV, VSV and Poliovirus with different doses of UV light. Log reduction of HSV with UV doses of 0.82, 1.63, 2.44 and 3.25 J/cm2 was 0.5, 1.3, 3.5 and 3.8, respectively. Log reduction of VSV for 0.82, 1.63, 2.44 and 3.25 J/cm2was 2.8, 3.8, 4.6 and 5.6, respectively. Also, log reduction of poliovirus for 0.82, 1.63, 2.44 and 3.25 J/cm2was 1.7, 2.5, 2.7 and 3.1 respectively. Conclusion: The final dose of UV (3.25 J/cm2) resulted in a larger amount of viral inactivation for enveloped and non-enveloped viruses, suspended in PLT. This method offers a potential to be used for prevention of the majority of PLT transfusion-associated viral pathogens.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Induction of Nucleic Acid Damage in Viral Genomes using Riboflavin in Combination with UV Light

Background and Aims: Despite the screening of blood donors, blood transfusion represents an ideal port of entry for blood-borne infection. Blood-borne pathogen transmission has been a concern since the earliest days of transfusion. The blood product of platelet (PLT) concentrates is still faced with the risk of bacterial and viral contaminations. Pathogen inactivation technologies offer a proac...

متن کامل

induction of nucleic acid damage in viral genomes using riboflavin in combination with uv light

background and aims: despite the screening of blood donors, blood transfusion represents an ideal port of entry for blood-borne infection. blood-borne pathogen transmission has been a concern since the earliest days of transfusion. the blood product of platelet (plt) concentrates is still faced with the risk of bacterial and viral contaminations. pathogen inactivation technologies offer a proac...

متن کامل

Principles of quantitation of viral loads using nucleic acid sequence-based amplification in combination with homogeneous detection using molecular beacons.

For quantitative NASBA-based viral load assays using homogeneous detection with molecular beacons, such as the NucliSens EasyQ HIV-1 assay, a quantitation algorithm is required. During the amplification process there is a constant growth in the concentration of amplicons to which the beacon can bind while generating a fluorescence signal. The overall fluorescence curve contains kinetic informat...

متن کامل

gradual erasure of subjectivity: a study of samuel beckett’s trilogy in the light of postmodernism

ساموئل بکت بیشتر از هر نویسنده دیگری در نیم? دوم قرن بیستم با گفتارش زمان? ما را به آستان? از هم پاشیدگی کشانده است، آستانه ای که در آن مدرنیته با سرانجام گریزان اما غیرقابل اجتناب خود مواجه می شود. در این تحقیق روی مفهوم فردیت و محو آن در دوران پسامدرن تاکید شده و در طی آن سعی شده است که فردیت مدرن و پسامدرن در رمان های سه گانه بکت بررسی گردد. تحقیق حاضر یک بررسی کتابخانه ای و کیفی بر روی سه ر...

15 صفحه اول

In vitro efficacy of antifungal treatment using riboflavin/UV-A (365 nm) combination and amphotericin B.

PURPOSE To demonstrate the antimicrobial properties of riboflavin/UV-A (365 nm) against fungal pathogens. METHODS The antimicrobial properties of riboflavin/UV-A (365 nm), with or without previous treatment with amphotericin B, were tested on three groups of fungi selected from severe cases of keratomycosis: Candida albicans, Fusarium sp, and Aspergillus fumigatus. They were tested by using K...

متن کامل

romantic education:reading william wordsworths the prelude in the light of the history of ideas

عصر روشنگری زمان شکل گیری ایده های مدرن تربیتی- آموزشی بود اما تاکید بیش از اندازه ی دوشاخه مهم فلسفی زمان یعنی عقل گرایی و حس گرایی بر دقت و وضوح، انسان عصر روشنگری را نسبت به دیگر تواناییهایش نابینا کرده و موجب به وجود آمدن افرادی تک بعدی شد که افتخارعقلانیتشان، تاکید شان بر تجربه فردی، به مبارزه طلبیدن منطق نیاکانشان وافسون زدایی شان از دنیا وتمام آنچه با حواس پنجگانه قابل درک نبوده و یا در ...

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 6  شماره None

صفحات  27- 32

تاریخ انتشار 2012-11

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023